thesislog.net contact us

The role of calcium in the organization of fibrillin microfibrils

FEBS letters / v.336 no.2. 1993, pp.323-326

http://dx.doi.org/10.1016/0014-5793(93)80829-J

Author : Kielty, C.M. ; Shuttleworth, C.A.

Abstract : The microfibrillar glycoprotein fibrillin has a multidomain structure which contains forty-three epidermal growth factor-like motifs with calciumbinding consensus sequences. We have utilized intact microfibrils isolated from human dermal fibroblast cultures to investigate the putative influence of bound calcium on microfibrillar organization and integrity. Incubation with EDTA or EGTA rapidly resulted in gross disruption of microfibril morphology. The treatment induced disorganization of the interbead domains although the regular beaded arrangement was always apparent. These changes were readily reversible on replacing calcium, indicating that the treatment had not compromised microfibrillar integrity. The data localize calcium binding EGF-like repeats to the interbead domains and indicate that lateral packing of fibrillin monomers is calcium-dependent. This arrangement suggests how mutations in epidermal growth factor-like domains of fibrillin might cause the disruption in microfibril organization and interactions which underlies the clinical symptoms of some Marian syndrome patients.

Keyword : Fibrillin . Calcium . EGF motif . Microfibril

Two Tcp-1-related but highly divergent gene families exist in oat encoding prot..

Tcp-1-related sequences have been isolated from a cDNA library of etiolated 6-day-old oat (Aven..

Human ferritin H-chains can be obtained in non-assembled stable forms which hav..

We found conditions to obtain the Leu-169 rarr; Arg mutant of human ferritin H chain in a stabl..

Analysis of lactase processing in rabbit

The proteolytic processing of rabbit intestinal lactase-phlorizin-hydrolase (LPH) was studied b..

Molecular cloning of a putative homolog of proline/arginine-rich antibacterial ..

Screening of a porcine bone marrow cDNA library with a PCR-derived probe from rabbit LPS-bindin..

The vanadium chloroperoxidase from the fungus, Curvularia inaequalis - Evidenc..

The binding of vanadate to the novel vanadium chloroperoxidase from C. inaequalis was investiga..

Retinal oxidase is identical to aldehyde oxidase

Retinal oxidase (EC 1.2.3.11) and aldehyde oxidase (EC 1.2.3.1) were compared with respect to t..

Raf-1 is not a major upstream regulator of MAP kinases in rat fibroblasts

RCR cells are NRK clones in which Raf-1 production is blocked by the expression of an antisense..

Time-resolved study of tryptophan fluorescence in vesicle reconstituted cytochr..

Time-resolved study of fluorescence decay of the tryptophan residue in bovine cytochrome c oxid..

Adhesive interactions of neutrophils and leukotriene synthesis

Cell-substrate and cell-cell adhesion of neutrophils has been found to slow down the calcium io..

Protein kinase A modulates an endogenous calcium channel, but not the calcium-a..

In Xenopus oocytes, Ca 2+ influx through an endogenous voltage-gated Ca 2+ channel activate..