contact us

Human ferritin H-chains can be obtained in non-assembled stable forms which have ferroxidase activity

FEBS letters / v.336 no.2. 1993, pp.309-312

Author : Levi, S. ; Santambrogio, P. ; Albertini, A. ; Arosio, P.

Abstract : We found conditions to obtain the Leu-169 rarr; Arg mutant of human ferritin H chain in a stable and non-assembled state. The protein obtained is an oligomer of subunits with a high degree of structured conformation, and when concentrated it re-assembles into ferritin cages. Functional studies showed that (i) it promotes iron oxidation like the assembled ferritin, but at slower rate, (ii) it is readily precipitated by the oxidised iron unless apotransferrin or L-chain ferritin are added to sequester Fe(III). The results confirm that ferroxidase activity is located within the H-chain, and indicate that the cages of the fully assembled ferritins are important not only in maintaining iron in a soluble form, but also in eliciting the activity of the ferroxidase centres.

Keyword : Ferroxidase . Ferritin . Iron protein . Protein assembly

Analysis of lactase processing in rabbit

The proteolytic processing of rabbit intestinal lactase-phlorizin-hydrolase (LPH) was studied b..

Molecular cloning of a putative homolog of proline/arginine-rich antibacterial ..

Screening of a porcine bone marrow cDNA library with a PCR-derived probe from rabbit LPS-bindin..

The vanadium chloroperoxidase from the fungus, Curvularia inaequalis - Evidenc..

The binding of vanadate to the novel vanadium chloroperoxidase from C. inaequalis was investiga..

Retinal oxidase is identical to aldehyde oxidase

Retinal oxidase (EC and aldehyde oxidase (EC were compared with respect to t..

Raf-1 is not a major upstream regulator of MAP kinases in rat fibroblasts

RCR cells are NRK clones in which Raf-1 production is blocked by the expression of an antisense..

Time-resolved study of tryptophan fluorescence in vesicle reconstituted cytochr..

Time-resolved study of fluorescence decay of the tryptophan residue in bovine cytochrome c oxid..

Adhesive interactions of neutrophils and leukotriene synthesis

Cell-substrate and cell-cell adhesion of neutrophils has been found to slow down the calcium io..

Protein kinase A modulates an endogenous calcium channel, but not the calcium-a..

In Xenopus oocytes, Ca 2+ influx through an endogenous voltage-gated Ca 2+ channel activate..

PAF antagonists in foods: Isolation and identification of PAF antagonists in vi..

Koussissis, S.G. ; Semidalas, C.E. ; Antonopoulou, S. ; Kapoulas, V.,

Selective hydrogenation of fatty esters into unsaturated alcohols using rutheni..

Piccirilli, A. ; Barrault, J.,